WebFeb 14, 2012 · Chromogenic in situ hybridization (CISH) is fast becoming a well established technique for easy and sensitive determination of HER2 gene status in breast cancer. However, for the chromogenic... WebINIS Repository Search provides online access to one of the world's largest collections on the peaceful uses of nuclear science and technology. The International Nuclear Information System is operated by the IAEA in collaboration with over 150 members.
Mean HER2/CEN-17 ratio and signal numbers for all specimens.
WebFeb 14, 2012 · HER2 CISH staining was performed according to the manufactures instructions (Dako Denmark A/S) at the US reference laboratory. In short, specimens were subjected to heat-pre-treatment (microwave oven) and pepsin digestion at 37°C to prepare the tissue for probe hybridization. Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. CISH is similar to FISH in that they are both in situ hybridization techniques used to detect the presence or absence of sp… graphite metallic chevrolet
HER2 IQFISH pharmDx Agilent
WebThe chromogenic in situ hybridization (CISH) assay, designed to detect the amplification of the HER2 gene in formalin-fixed, paraffin-embedded (FFPE) breast cancer (BC) and gastric cancer (GC) tissue specimens, was evaluated in 125 FFPE BC cases and 198 FFPE GC cases for which the HER2 status had been predetermined using immunohistochemistry … WebAug 1, 2010 · The data obtained revealed an overall HER2 status concordance of approximately 98% for comparisons of HER2 CISH pharmDx™ Kit to both HER2 FISH pharmDx™ Kit and PathVysion HER-2 DNA Probe Kit. WebMar 2, 2024 · Table 3. Cross tabulation of HER2 gene status for CISH versus Dako FISH. HER2 FISH pharmDx™ Total; Non-amplified Amplified; HER2 CISH pharmDx™ Non … chisels me mod