Ctd110.6 antibody

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August undefined, 2024

WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Crossreactivity: WebJun 15, 2001 · The antibody reacts with a large number of proteins from cytoplasmic and nuclear extracts and readily detects in vivo changes in O-GlcNAc modification. These …

Anti-O-GlcNAc (CTD110.6) antibody Monoclonal Antibodies

WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87]. WebJul 15, 2014 · The antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought to accumulate in cells experiencing severe glucose deprivation. In this study, we have addressed two questions: (1) Which other antibodies … csd bad hersfeld

Characterization of a mouse monoclonal antibody specific for O …

WebBinding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell surface … WebAntibody: Clone: CTD110.6: Immunogen : This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b … Webwww.ncbi.nlm.nih.gov csdb botswana

Anti-O-GlcNAc Antibody, clone CTD110.6 - Sigma-Aldrich

WebWestern blotting of SDS-PAGE gels with O-GlcNAc-specific antibodies (RL2 and CTD110.6) shows that platelets contain 15-20 major O-GlcNAcylated proteins. Upon stimulation by agonists ( e.g. thrombin, TRAP, collagen, U46619, and A23187), the extent of modification displays protein- and agonist-specific changes. Treatment of platelets with … WebNov 22, 2024 · Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of … csd barnechea vs cd magallanesWebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at … dyson flyaway attachment hair

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Ctd110.6 antibody

Frontiers New Insights Into the Biology of Protein O …

WebDec 11, 2009 · The following antibodies were used: anti-O-GlcNAc (CTD110.6, Covance), anti-OGT (DM17, Sigma), anti-actin, anti-glycogen phosphorylase ... Korea) for providing the glutamine:fructose-6-phosphate amidotransferase antibody. Supplementary Material. Download : Download Acrobat PDF file (541KB) Recommended articles. REFERENCES … WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

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WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... WebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and …

WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t … WebDec 9, 2016 · The RL2 epitope was detected on Sp1 during 2DG treatment, and the level was transiently increased at 24 h. In contrast, the CTD110.6 epitope became detectable on Sp1 over 72 h after 2DG treatment, and then the other proteins containing CTD110.6 epitopes also appeared in the cell lysates and the anti-Sp1 antibody precipitates.

WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation. WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 …

WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit

WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O … csd autism gilroyWebApr 18, 2014 · Binding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell … dyson fluffy on carpetWebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). csd batteryWebAnti-O-GlcNAc Antibody, clone CTD110.6 clone CTD110.6, from mouse; Synonyms: O-GlcNAc, beta-O-GlcNAc, O-Linked N-Acetylglucosamine, beta-O-linked N … dyson flyaway attachment buyWebMonoclonal Antibody for studying Ser/Thr O-GlcNAc. Cited in 70 publications. Validated for Western Blotting. Highly specific and … dyson flyaway hair dryer attachmentWebTime course studies by western blot with the CTD110.6 antibody demonstrated that i.v. injection in rat at 50 mg/kg increased global protein O-GlcNAcylation in total brain homogenates, starting at 1 h and peaking at 10 h and decreased pS396 tau/Tau-5 starting at 1 h, peaking at 4 h, and restoring to normal at 16 h (Yuzwa et al., 2008). csdb c64 last releaseWebOct 11, 2013 · For immunodetection, CTD110.6 antibody was used because it is widely used for the detection of O-GlcNAcylated proteins. Compared to the α-DG-Fc isolated from the control transfectants, immunoreactivity of α-DG-Fc towards CTD110.6 was elevated upon GTDC2 overexpression (Fig. 1A). cs davidson jobs york pa