Phosphinothricin-resistant
WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebPhosphinothricin is not an effective antibiotic because of its inability to be readily …
Phosphinothricin-resistant
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WebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … WebA bioassay was developed for identifying transgenic wheat plants based on De Block et …
WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by … WebThese results indicated that the extra intron at the 5 0 end of the phosphinothricin-resistance gene is required for its efficient expression in G. lucidum. To confirm these results, we also ...
Glufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring herbicides, directly into glufosinate. The … WebNov 6, 2006 · The phosphinothricin-resistant seedlings were uniformly much greener than …
WebOct 21, 2024 · The CRISPR vector for this study was used in a previously published pBAtC that is composed of the U6 promoter from Arabidopsis to express gRNA including CRISPR RNA scaffold, the 35S promoter originally from cauliflower mosaic virus (CaMV) to express Cas9 and the Basta TM (Phosphinothricin) resistance gene for the selection of the first ...
WebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces … the president\u0027s 2012 executive order 13627 onWebJan 1, 2008 · Phosphinothricin resistance as selectable marker for genetic transformation … sigh meaning in chatWebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... sigh mailWebOct 23, 2015 · Increasing PPT-resistant gene diversity and generating multi-herbicide … the president\u0027s challenge fitness testWebOct 1, 1996 · The enzymes have a similar substrate affinity towards L-phosphinothricin and do not acetylate any of the other L-amino acids tested. ... Engineering herbicide resistance in plants by expression of ... sighmathWebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related … the president\u0027s chief executiveWebLibertyLink (®) cotton cultivars are engineered for glufosinate resistance by … sigh mall