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pLVX-shRNA2说明书-分析方法-生物在线 Lab-on-Web - BIOON
WebPlasmid pLVX-M-puro from Dr. Boyi Gan's lab is published in Nat Cell Biol. 2024 Oct;20(10):1181-1192. doi: 10.1038/s41556-018-0178-0. Epub 2024 Sep 10. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view ... Web质粒pLVX-shRNA2-Puro回收大片段与shRNA-目的基因的稀释退火产物的连接,22℃水浴反应过夜。. 连接反应体系如下:. 5. 各取10 μl过夜连接产物转化100 μl Stbl3感受态细胞: … hometalon myynti
Optimized protocol for high-titer lentivirus production and ...
WebSep 20, 2024 · pLVX-TetOne系列质粒载体是集调控与应答于一体的四环素诱导型慢病毒载体. HH-LV-098 pLVX-TetOne调控应答一体四环素诱导型慢病毒载体 无筛选标记. HH-LV-100 pLVX-TetOne-BSD调控应答一体四环素诱导型慢病毒载体blast杀稻瘟菌素. HH-LV-101 pLVX-TetOne-EGFP调控应答一体四环素诱导 ... WebThe System contains an HIV-1-based, lentiviral expression vector, pLVX-EF1α-IRES-Puro, designed to constitutively express a protein of interest and puromycin resistance (Puro r). Constitutive expression of these proteins is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows protein expression in mammalian cells in ... WebJun 1, 2024 · 描述. pLVX-shRNA1 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is drivenby the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA1 can either be used as a plasmid expression vector … home tallahassee