Webb11 jan. 2024 · For each sample, 1 μg of total proteins was loaded onto a 4–20% polyacrylamide TGX pre-cast gel (Bio-Rad), together with 3 μL of Sharpmass VII Prestained Protein Marker (EuroClone, Milan, Italy). The same procedure with slight modifications was used to evaluate the expression of cathepsin L in ovaries dissected from S. titanus … Webb15 dec. 2024 · SharpMass™ 1-kb Ready-to-load DNA Ladder and SharpMass™ 100–100-bp DNA Ladder (EuroClone, Milan, Italy) were utilized as DNA size markers. Gel images were visualized using Chemidoc XRS (Bio-Rad). Purification of DNA from agarose was carried out using Wizard SV gels and PCR Clean-UP system ...
SHARPMASS™ 100 - 100 lanes - Euroclone group
Webb12 juni 2024 · Nucleic Acids Electrophoresis - Euroclone group · PDF file Nucleic Acids Electrophoresis 3 17 GellyPhor® HR GellyPhor® HR is a molecular biology grade standard melting temperature; Match case Limit results 1 per page. Click here to load reader. Post on 12-Jun-2024. 3 views. Category: Documents. 0 download. Report. Download; Facebook. Webb15 jan. 2014 · After SDS-polyacrylamide gel electrophoresis (PAGE), proteins were transferred to nitrocellulose membrane (Whatman GmbH, Dassel, Germany) and cut with scissor at 70 kDa molecular weight based on molecular weight markers (Prestained Protein SHARPMASS V PLUS, Euroclone, Milan, Italy). sifma cybersecurity
Veterinary Pathology Felis catus Papillomavirus Types 1, 2, 3, 4, …
Webb18 aug. 2024 · It catalyses intracellular reactions of Ca 2+ -dependent transamidation or of hydrolysis of GTP, as a G-protein interacting with transmembrane receptors in cell signalling, whereas outside the cell this enzyme promotes the stabilization of the ECM (Extracellular Matrix) [ 1, 2, 3, 4 ]. WebbSharpMass™ 100 Plus Ladder (Euroclone, Milano, Italy) was used as a DNA marker. PCR products of the expected length and with a sufficient concentration were forward and reverse Sanger sequenced by an external company. WebbGenotype was determined by fragment sizes: 484 bp (S, short allele) and 528 bp (L, long allele). 100 bp SHARPMASS (Euroclone) was used as DNA ladder. 2.8. Statistical Analysis Three experiments performed in duplicate were performed for each analysis. Data are expressed as mean ± SD and -test was used for statistical analysis. 3. sifma early close 2021