T4 ligase hc
WebT4 DNA Ligase (Rapid) Description [600,000 Units/ml] 240,000 Units (0.40 ml) Share your knowledge of this product with other customers... Be the first to write a review Browse for more products in the same category as this item: Ultrapure Ligases WebThe T4 DNA Ligase requires ATP as a cofactor. Highlights. Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky …
T4 ligase hc
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Web10 µL 2X Rapid Ligase Buffer X Vector (1 -10 ng/µL ) Y Insert (1 -10 ng/µL ) 1 µL T4 DNA Ligase ( 10 U/ µL) To 20 µL Nuclease -free Water . 2. Mix well by pipetting. 3. Incubate at 25 °C for 10 minutes 4. Purify DNA using a PCR clean-up column and elute in ~50 µL. – OR – Immediately dilute in TE or water (at least 1:10). Web10X T4 DNA Ligase Buffer (B603) 500mM Tris-HCI 100 mM MgCl 2 50 mM dithiothreitol 10 mM ATP pH 7.6 @ 25°C Molecular Weight 55.3 kDa Unit Definition 1 unit is defined as …
Web搜索到 25 条记录 . 分类. 突变 (1) 克隆试剂盒 WebT4 DNA Ligase ( NEB #M0202) Is the enzyme of choice for the majority of recombinant DNA applications. It can be used for for cohesive ends (10 mins RT) and blunt ends (2 …
WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. WebJun 8, 2016 · High performance of T4 ligase is depending on integrity of the ATP in buffer. to keep the balance between T4 ligase optimal activity temperature (25 c) and ATP hydrolysis issue also cross...
WebMar 30, 2024 · The key point is the design of L-RNA substrate with appropriate reactivity for T4 Rnl2 ligation, which is efficient enough for intramolecular circularization but not reactive for intermolecular ligation. First, the secondary structure of target C-RNA is determined by Mfold prediction ( 40, 41 ).
WebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3' hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. is a little bit of alcohol badWebT4 RNA Ligase 1 Add to Cart: L6050L: 10,000 U: 20,000 U/ml: $443: Single-stranded RNA ligase. Also joins single-stranded DNA molecules. Add to Cart: T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 DNA Ligase Add to Cart ... olive oil for cooking best brandWebRNA Circularization using T4 RNA Ligase 1 Protocol for ssDNA/RNA Ligation (M0319) Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1 3´ End Labeling of RNA using T4 RNA Ligase 1 Typical Protocol for RNA Ligation (M0458) Featured Products T4 RNA Ligase 2, truncated K227Q T4 RNA Ligase 1 (ssRNA Ligase) olive oil for dandruff and itchy scalpWebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate … olive oil for constipation in dogsT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky-end ligation is completed in 10 minutes at room temperature • Supplied with PEG solution for efficient blunt-end ligation Applications is a little bit of alcohol good for youWebT4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. ... T4 DNA Ligase (HC) M179A: 1 × 500u: … olive oil for diarrheais alito married